With the help from Scion, the following program modifies NIH Image 1.50b83 and has several features (with Scion LG-3 board): The first change to look at is the Make Movie command. The dialog box structure has been changed. There is only one box for entering the number of frames and the frame delay. There are two check boxes: one to activate video rate capturing and the other to activate blind movie capturing. A macro command MakeMovie has been added, which optionally takes three arguments. The first argument is a string which may contain some combination of 'Blind', 'VideoRate', and 'DacControl'. The first two strings activate the same modes as the check boxes mentioned above. 'DacControl' enables control of the digital to analog converters during movie capturing. This feature will only work if video rate capturing is enabled. The second argument is the number of frames to capture. The third argument is the delay between frames. Note that if video rate capturing is specified, the frame delay will always be taken as zero. You may specify how the dacs are to be manipulated during capture using the new macro command, StoreScion(frame: integer, dacA: integer, dacB: integer,delay:integer). Frame may be from 1 to 128, the dac's may range from 0 to 255. Delay can be from 0 to 255. When this command is used, it will cause the dac's to be set to the given values prior to capture of the specified frame during a MakeMovie command (provide that 'DacControl' is specified. After the Dacs are set prior to the specified frame the software will wait for delay frames before recommencing video rate capturing. All previous dac specifications can be cleared (set to zero) using the macro command ScionClear. For example, suppose you wish to capture 32 frames at video rates with the following dac specifications: Before frame 1 dacA 37 dacB 221 Skip 2 frams Before frame 19 dacA 147 dacB 0 Skip 3 frams Before frame 23 dacA 12 dacB 178 Then you could use the following macro macro 'Example'; {for video rate capture} begin ClearScion; StoreScion(1,37,221,0); StoreScion(19,147,2); StoreScion(23,12,178,3); MakeMovie('VideoRate DacControl',32,0.0); end; ------------- Guang Mei gmei@hoh.mbl.edu Tel:(508)548-3705 ext 374 Program of Architectural Dynamics in Living Cells Marine Biological Laboratory, Woods Hole, MA 02543-1031